With her dark sunglasses and stony expression, the image of Hillary staring at her smartphone made her look such a boss that it launched its own Tumblr parody account.
The photograph, which dates from a trip to Tripoli, Libya in September 2011, was even adopted by the former Secretary of State herself for her Twitter profile picture.
However, it seems that the picture which inspired hundreds of comedy memes could be no laughing matter for Clinton as it emerges the photograph could be a figurative smoking gun.
Indeed, Clinton herself has removed the image removed from her Twitter account because it could show her using her controversial personal email account to conduct diplomacy in Libya.
For more than a year, the former Secretary of State had used the monochrome image of her using a Blackberry on board a C-military plane headed to Tripoli in September 2011, around a month after Colonel Gaddafi's regime fell.
But the picture, regularly seen by her 2.9million followers, was removed Sunday - ostensibly to promote a political campaign for gender equality, timed to coincide with International Women's Day.
The image was once a symbol of Clinton's funny side as the face of the Texts From Hillary meme, which she said had given her 'many LOLZ' - but fell out of favor this week.